bs 17668r  (Bioss)

Journal: Oncogenesis

Article Title: Human papillomavirus16 E7 enhances cell stemness by regulating the APC2/SPIN4/β-catenin axis in cervical cancer

doi: 10.1038/s41389-026-00602-8

Figure Lengend Snippet: A Venn diagram showing the overlap of HPV16 E7 silence-related DEGs and TCGA data of APC2-related DEGs in cervical cancer. (B) FPKM value of these overlap genes (** p < 0.01, * p < 0.05). C The bar graph represents the qPCR analysis of these overlap DEG expressions in siR-E7-treated SiHa cells ( n = 3; student’s t -test; ** p < 0.01, * p < 0.05). D WB analysis of SPIN4 expression in Caski and SiHa cells treated with siR-NC or siR-E7 ( n = 3; student’s t -test; ** p < 0.01, * p < 0.05). E Correlation analysis between APC2 and SPIN4 expression in cervical cancer tissues. A scatter plot with the correlation coefficient ( R ) and p -value is shown. F The expression of SPIN4 in clinical samples was detected by IHC assay. (200× and 400× magnification; Scale bar: 100 μm and 50 μm; n = 10; student’s t -test; ** p < 0.01). G The expression of SPIN4 in HPV16 E7-silenced xenograft tumor tissues was detected by IHC assay. (200× and 400× magnification; Scale bar: 100 μm and 50 μm; n = 3; student’s t -test; * p < 0.05). H WB analysis of SPIN4 expression in cervical cancer cells transfected with APC2 overexpression or APC2 silencing ( n = 3; student’s t-test; ** p < 0.01, * p < 0.05).

Article Snippet: SPIN4 , Bioss , bs-17668R , 1:2000 (WB) 1:200 (IHC).

Techniques: Expressing, Transfection, Over Expression

Journal: Oncogenesis

Article Title: Human papillomavirus16 E7 enhances cell stemness by regulating the APC2/SPIN4/β-catenin axis in cervical cancer

doi: 10.1038/s41389-026-00602-8

Figure Lengend Snippet: A Box plot comparing SPIN4 expression levels between adjacent healthy and tumor tissues in cervical cancer patients from the TCGA database. p = 0.027 by the Wilcoxon signed-rank test. B Kaplan–Meier curve illustrating the overall survival of cervical cancer patients stratified by high (blue line) or Low (red line) SPIN4 expression. Using C GO enrichment analysis and D KEGG methods, we evaluated the functional categories and potential signaling pathways enriched by SPIN4-related DEGs. E SiHa cells were transfected with siR-SPIN4 or siR-NC, and the transfection efficiency was examined by WB assay (n = 3; student’s t -test; ** p < 0.01). F Proliferation ability of SiHa cells transfected with pcDNA3.1+siR-NC, APC2+siR-NC, or APC2+siR-SPIN4 was assessed by MTT assay at 0, 24, 48, and 72 h, respectively ( n = 3; ANOVA; ** p < 0.01). G The transwell assay evaluated the impact of APC2 overexpression combined with SPIN4 silencing on SiHa cell migration and invasion, contrasted with control cells. The image was magnified 100× (Scale bar: 100 μm; n = 3; ANOVA; ** p < 0.01, * p < 0.05). H S p here-forming assay showing the effects of SPIN4 silencing on sphere formation in SiHa cells with APC2 overexpression (Scale bar: 50 μm; n = 3; ANOVA; ** p < 0.01). I The protein levels of OCT4, SOX2, Nanog, and β-catenin expressed in SiHa cells that overexpressed APC2 and simultaneously silenced SPIN4 were tested by WB assays ( n = 3; ANOVA; ** p < 0.01, * p < 0.05).

Article Snippet: SPIN4 , Bioss , bs-17668R , 1:2000 (WB) 1:200 (IHC).

Techniques: Expressing, Functional Assay, Protein-Protein interactions, Transfection, MTT Assay, Transwell Assay, Over Expression, Migration, Control

Journal: Oncogenesis

Article Title: Human papillomavirus16 E7 enhances cell stemness by regulating the APC2/SPIN4/β-catenin axis in cervical cancer

doi: 10.1038/s41389-026-00602-8

Figure Lengend Snippet: A Representative images of xenograft tumors harvested from mice injected with SiHa cells stably expressing either non-targeting control shRNA (Lenti-sh-NC) or SPIN4-targeting shRNA (Lenti-sh-SPIN4) ( n = 3). B Mouse body weights were monitored throughout the experiment. C Quantitative analysis of tumor volume and weight ( n = 6; student’s t -test; ** p < 0.01). D IHC staining of xenograft tumor sections for the stemness markers OCT4, SOX2, and Nanog, and the Wnt pathway component β-catenin ( n = 3; student’s t -test; ** p < 0.01).

Article Snippet: SPIN4 , Bioss , bs-17668R , 1:2000 (WB) 1:200 (IHC).

Techniques: Injection, Stable Transfection, Expressing, Control, shRNA, Immunohistochemistry